Research Summary

Our research program focuses on the characterization of signaling pathways and cellular organelles in the heart, with a particular interest on alterations of subproteomes during myocardial ischemic injury. Since 1992, this research program has authored 90 manuscripts in cardiac cell signaling and myocardial ischemic injury. The functional proteomic approaches we employ have provided key information pertaining to the proteomic basis of diseased cardiac phenotypes, and enabled a holistic portrait of multiple molecules and pathways in parallel. The proteomic delineation combined with genetic interrogations has been effective to gain pathogenic insights in heart diseases. Dr. Peipei Ping, the Director of our research program, is the principal investigator of an NHLBI Program Project Award on Myocardial Ischemic Injury and Protection. For additional information please visit our website at www.signalingmodules.org.

Representative Publications

Lu H, Zong C, Wang Y, Young GW, Deng N, Souda P, Li X, Whitelegge J, Drews O, Yang PY, Ping P. "Revealing the dynamics of 20S proteasome phosphoproteome: A combined CID and ETD approach." Mol Cell Proteomics. (In Press) (2008)

Zhang J, Li X, MuJames N. Weiss, Paavo Korge, Henry M. Honda, Peipei Pingeller M, Wang YJ, Zong C, Deng N, Vondriska TM, Liem DA, Yang YJ, Korge P, Honda H, Weiss JN, Apweiler R, Ping P. “Systematic Characterization of the Murine Mitochondrial Proteome Using Functionally Validated Cardiac Mitochondria”. Proteomics 8(8):1564-1575 (2008)

Zhang J, Liem DA, Mueller M, Wang YJ, Zong C, Deng D, Vondriska TM, Korge P, Drews O, MacLellan WR, Honda H, Weiss JN, Apweiler R, Ping P. “Altered Proteome Biology of Cardiac Mitochondria Under Stress Conditions”. J Proteome Res. (In Press) (2008)

Taylor CF, Paton NW, Lilley KS, Binz PA, Julian RK Jr, Jones AR, Zhu W, Apweiler R, Aebersold R, Deutsch EW, Dunn MJ, Heck AJ, Leitner A, Macht M, Mann M, Martens L, Neubert TA, Patterson SD, Ping P, Seymour SL, Souda P, Tsugita A, Vandekerckhove J, Vondriska TM, Whitelegge JP, Wilkins MR, Xenarios I, Yates JR 3rd, Hermjakob H. “The Minimum Information about a Proteomics Experiment (MIAPE)”. Nat Biotechnol., 25: 887-893 (2007)

Drews O, Wildgruber R, Zong C, Sukop U, Nissum M, Weber G, Gomes AV, Ping P. “Mammalian Proteasome Subpopulations with Distinct Molecular Compositions and Proteolytic Activities”. Mol Cell Proteomics., 6: 2021-2031 (2007)

Gomes AV, Zong C, Edmondson RD, Li X, Stefani E, Zhang J, Jones RC, Thyparambil S, Wang GW, Qiao X, Bardag-Gorce F, Ping P. “Mapping the Murine Cardiac 26S Proteasome Complexes”. Circ Res., 99: 362-371 (2006)

Zong C, Gomes AV, Drews O, Li X, Young GW, Berhane B, Qiao X, French SW, Bardag-Gorce F, Ping P. “Regulation of Murine Cardiac 20S Proteasomes: Role of Associating Partners”. Circ Res., 99: 372-380 (2006)

Weiss JN, Korge P, Honda HM, Ping P. “Role of the Mitochondrial Permeability Transition in Myocardial Disease”. Circ Res., 93: 292-301 (2003)

Edmondson RD, Vondriska TM, Biederman KJ, Zhang J, Jones RC, Zheng Y, Allen DL, Xiu JX, Cardwell EM, Pisano MR, Ping P. PKC signaling complexes include metabolism- and transcription/translation-related proteins: complimentary separation techniques with liquid chromatography and tandem mass spectrometry. Mol Cell Proteomics. 2002;1:421-433.

Baines PC, Zhang J, Wang GW, Zheng YT, Xiu JX, Cardwell EM, Bolli R, Ping P. Mitochondrial PKC and MAPK form signaling modules in the murine heart. Circ Res. 2002;90:390-397.

Balafanova Z, Bolli R, Pass JM, Wang O, Zhang J, Gao J, Bhatnagar A, Ping P. Nitric oxide donors induce nitration of PKC , facilitating PKC translocation via enhanced PKC -RACK2 interactions: a novel mechanism of NO-triggered activation of PKC. J Biol Chem 2002;277:15021-15027.

Ping P, Song C, Zhang J, Guo Y, Cao X, Li R, Wu W, Vondriska TM, Pass JM, Tang XL, Pierce WM, Bolli R. Formation of PKC -Lck signaling modules confers cardioprotection. J Clin Invest 2002;109:499-507.

Song C, Vondriska TM, Wang GW, Klein JB, Cao X, Zhang J, Kang Y, D'Souza S, Ping P. Molecular conformation dictates module formation: example of PKC and Src tyrosine kinase. Am J Physiol 2002;282:H1166-H1171.